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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/851
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dc.contributor.authorJana, Satavisa-
dc.date.accessioned2017-10-24T07:07:52Z-
dc.date.available2017-10-24T07:07:52Z-
dc.date.issued2017-07-13-
dc.identifier.urihttp://hdl.handle.net/123456789/851-
dc.description.abstractTrapping of reaction intermediates has been used as an approach to study the mechanism of various reactions. It serves the purpose of providing a convincing proof as to whether a particular reaction is going via a certain pathway. Here we trap and study an intermediate that is formed during the enzymatic reaction of Sortase A using the help of Force Spectroscopy via Atomic Force Microscopy (AFM). We estimate the lifetime and the off rates of the formed intermediate.en_US
dc.description.sponsorshipIISER-Men_US
dc.language.isoenen_US
dc.publisherIISER-Men_US
dc.subjectChemistryen_US
dc.subjectSpectroscopyen_US
dc.subjectSortase Aen_US
dc.subjectAtomic Force Microscopyen_US
dc.titleIntermediate Trapping Using Force Spectroscopyen_US
dc.typeThesisen_US
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