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http://hdl.handle.net/123456789/851| Title: | Intermediate Trapping Using Force Spectroscopy |
| Authors: | Jana, Satavisa |
| Keywords: | Chemistry Spectroscopy Sortase A Atomic Force Microscopy |
| Issue Date: | 13-Jul-2017 |
| Publisher: | IISER-M |
| Abstract: | Trapping of reaction intermediates has been used as an approach to study the mechanism of various reactions. It serves the purpose of providing a convincing proof as to whether a particular reaction is going via a certain pathway. Here we trap and study an intermediate that is formed during the enzymatic reaction of Sortase A using the help of Force Spectroscopy via Atomic Force Microscopy (AFM). We estimate the lifetime and the off rates of the formed intermediate. |
| URI: | http://hdl.handle.net/123456789/851 |
| Appears in Collections: | MS-12 |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| MS12069.pdf | 19.87 kB | Adobe PDF | View/Open |
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