Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1728
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dc.contributor.authorLata, Hem-
dc.contributor.authorPatil, Sunil A.-
dc.date.accessioned2021-12-13T05:27:29Z-
dc.date.available2021-12-13T05:27:29Z-
dc.date.issued2020-06-
dc.identifier.urihttp://hdl.handle.net/123456789/1728-
dc.description.abstractChemolithoautotrophic microorganisms play an important role in carbon and energy metabolism in different environments through the fixation of CO2 using inorganic energy sources such as hydrogen (H2) and hydrogen sulfide (H2S). These microbes are also of interest for the development of CO2-based bioproduction technologies. Acetogenic bacteria belong to this group. These are obligate anaerobes that reduce CO2 into acetate and conserve energy via the Wood–Ljungdahl pathway (WLP). These microorganisms have been reported from various natural but mostly normal environments. Not much is known about their ecology and diversity in extreme environments, such as saline soda lakes. Such environments host unique microbial communities that possess exceptional metabolic capabilities due to their adaptation to harsh growth conditions. In the present study, intending to improve our understanding of CO2 fixing microorganisms in extreme environments, I attempted the enrichment of chemolithoautotrophic bacteria from a highly saline and alkaline environment of the Lonar lake with CO2 and H2 as the only carbon and energy sources, respectively. The experiments were conducted under 2% salinity and 7 or 9.6 pH conditions. The enrichment of chemolithotrophs was tracked by monitoring the growth via OD measurements and the production of organic acids via HPLC. At pH 7, the production of acetic acid (467.4 ± 7.4 mg/L) and formic acid (307.8 ± 3.4 mg/L) suggested successful enrichment of the CO2 fixing chemolithotrophs. In the case of pH 9.6 condition, only formic acid (88.9 ± 1.3 mg/L) production was observed. It suggests the enrichment of novel microbes, which are capable of growing and conserving energy by fixing CO2 and H2 into only formic acid. It is an interesting observation, which warrants further investigations. A unique growth pattern comprising of black colored aggregates and thread-like structures was observed in the culture flasks. Microscopic characterization revealed the presence of oval-shaped bacteria aligned in long chains in these structures. In conclusion, successful enrichment of the chemolithotrophic microorganisms was achieved from the sediment samples of an extreme haloalkaline environment in this work. Further characterization through metagenomics is expected to reveal the most abundant CO2 fixing lithotrophs in the enriched cultures.en_US
dc.language.isoenen_US
dc.publisherIISERMen_US
dc.subjectMicrobial CO2 fixationen_US
dc.subjectEnrichment of CO2 fixing bacteriaen_US
dc.subjectlithotrophsen_US
dc.subjectLonar lakeen_US
dc.titleCO2 fixing lithotrophs from a highly haloalkaline environment of the Lonar lakeen_US
dc.typeThesisen_US
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