http://hdl.handle.net/123456789/1324 Dissertation submitted by MP -2016 batch as part of their course. 2023-06-05T03:07:58Z http://hdl.handle.net/123456789/1846 Title: Free Subgroups in the Unit Group of Group Rings Authors: Mittal, Shubham; Passi, I. B. S. 2019-04-25T00:00:00Z http://hdl.handle.net/123456789/1115 Title: To elucidate the role of Arl8b interaction partner RUFY3 in regulating lysosome positioning Authors: Chawla, Prateek; Sharma, Mahak Abstract: Recent advancements in the field of lysosome biology have uncovered that in addition to its degradative function, lysosomes play an essential role in regulating several key biological processes such as plasma membrane repair, antigen presentation, cell migration/invasion and metabolic signalling. It has become increasingly evident that cellular distribution of lysosomes plays an essential role in regulating most of these unconventional functions. Researchers have identified several molecular players, which efficiently regulate lysosome positioning in the cell. Interestingly, small GTPases have emerged as one of the key players in the process. Our group has a keen interest in understanding the role of lysosomal small GTP binding protein Arl8b and its effectors in regulating lysosome positioning and cargo trafficking. As part of my master’s thesis work, I am characterizing anovel interaction partner of Arl8b, which is a RUN domain containing protein and elucidating the significance of these protein-protein interactions on cargo transport towards lysosomes. 2019-11-18T00:00:00Z http://hdl.handle.net/123456789/1093 Title: Investigating post-translational regulation by glutathionylation of glycolytic pathway enzymes of Saccharomyces cerevisiae Authors: Mishra, Vibhu; Bacchawat, Anand Kumar Abstract: Glycolysis is the pathway that consumes glucose to produce energy. It is present in all organisms, from bacteria in the deep oceans to complex life forms present on the earth. During oxidative stress, the cell shifts the glycolytic pathway flux into Pentose Phosphate pathway to generate NADPH to be used by glutaredoxins and thioredoxins to deal with oxidative stress. For this to happen, the flux through the glycolytic pathway has to be shut off by regulation of enzymes of the glycolysis. This has to happen quickly to deal with damaging oxidative stress, since the enzymes’ thiols may get permanently oxidised and degraded. So, the cell needs a mechanism that can regulate as well as protect the protein cysteine thiol from permanent oxidation.Glutathionylation is such a mechanism. Several reports have shown that many glycolytic proteins undergo glutathionylation under cellular oxidative stress. Glutathionylation prevents irreversible oxidation of cysteine residues present in proteins by formation of mixed disulphides. However, the mechanistic role of glutathionylation of these glycolytic proteins has not been well studied. In the current study, we tried to investigate this process in detail under stress and non-stress conditions.From the literature there are six enzymes known to be glutathionylated in yeast. These enzymes are Glyceraldehyde-3-phosphate dehydrogenase (TDH3), Fructose-bisphosphate aldolase (FBA1), 3-Phosphoglycerate Kinase (PGK1), Enolase (ENO2), Pyruvate Kinase (CDC19) and Triose Phosphate Isomerase (TPI1). To initiate investigations on the project, all six genes were cloned and expressed downstream of a strong yeast promoter. The expression of three of these enzymes Fba1p, Pgk1p, Eno2p could be confirmed. We then evaluated glutathionylation of these enzymes under stressed and non-stressed conditions. To investigate the enzyme involved in the glutathionylation and deglutathionylation process, we examined glutathionylation or deglutathionylation in genetic backgrounds deleted for the different thioredoxins and glutaredoxins that are known to be involved in these pathways. Preliminary results indicate a role of Grx3p in the glutathionylation of Fba1p, Grx1p in the glutathionylation of Pgk1p and Trx2p in the deglutathionylation of Pgk1p. However, more detailed studies are required to confirm these initial findings and determine the mechanisms involved. 2019-11-15T00:00:00Z http://hdl.handle.net/123456789/1092 Title: ISOTHERMAL COMPRESSIBILITY AND SPECIFIC HEAT OF HADRONIC MATTER FORMED IN HEAVY-ION COLLISIONS Authors: Doomra, Vassu; Jena, Satyajit Abstract: According to big-bang theory, at the earliest of its expansion, the universe existed as Quark- Gluon-Plasma (QGP). As it cooled down, the deconfinement-confinement phase transition occurred, and hadrons were formed. A study of this phase transition can lead us to understand the early stages of universe formation. The transformation of matter at high enough energies, from nucleons to constituent quarks and gluons had been very fascinating and equally very challenging. In this thesis, we intend to study ultra-relativistic heavy-ion collisions by using fluctuations of charged particle multiplicity and temperature. The study of event-by-event fluctuations of temperature and charged particle multiplicity will give an approximation of the specific heat and isothermal compressibility of the system respectively. A novel method has also been proposed for the specific heat calculation based on the lattice QCD (Quantum Chromodynamics) simulation results on the initial energy density in heavy-ion collisions. Together these two observables can predict something about the critical point in the QCD phase diagram. The temperature parameter is obtained from the transverse momentum distribution of the outgoing particles. Several models are studied for obtaining the best approximation to the experimental data on transverse momentum of identified particles from Pb + Pb collisions at p sNN = 2.76 TeV. Charged particle multiplicity distributions are studied for Au + Au collisions at p sNN = 200 GeV and the variation of isothermal compressibility is shown over a temperature range of 150 MeV to 250 MeV. 2019-11-15T00:00:00Z