http://hdl.handle.net/123456789/1322 Dissertation submitted by MP -2014 batch as part of their course. 2026-04-26T06:21:18Z http://hdl.handle.net/123456789/1024 Title: Some Topics in Algebraic Topology Authors: Upreti, Manoj Abstract: There is no abstract 2018-11-28T00:00:00Z http://hdl.handle.net/123456789/863 Title: Understanding the Role of Wnt Signaling in Context of Zebrafish Fin Regeneration Authors: Sharma, Priya; Ramachandran, Rajesh Abstract: Wnt signaling is known to be involved in retina regeneration but its importance in fin regeneration has not been studied yet. LiCl is a positive regulator of Wnt signaling pathway by inhibiting GSK3β. So, we studied the effect of LiCl on zebrafish caudal fin regeneration and we found that LiCl acts a positive regulator of caudal fin regeneration by upregulatingWnt signaling pathway as blastema regeneration has increased from 125μM to 500μM. Along with this, we studied the effect of tumour suppressor gene Ptenb inhibitor and EDTA on fin regeneration and we found that both of these downregulates fin regeneration whereas HDACs inhibitor, LiCl and CaCl2 upregulates fin regeneration. Then, we studied the effect of Lithium ions on embryos which revealed that embryos showed delayed hatching at 8dpf in the presence of Li ions as compared to the control as Lithium upregulates Wnt signaling which further downregulates hyaluronidase enzyme due to which chorion does not break down. Then, we studied about pluripotency factors where there is induction of pluripotency factors during retina regeneration, embryonic development and fin regeneration. mRNA in situ hybridization of pluripotency factors revealed their spatial expression pattern at different time points during embryonic development. Finally, we did immunostaining where it has been shown that notch inhibitor causes induction of sox2 during proliferation of astrocytes in mice retina and DAPT enhances proliferation of cells. 2017-07-18T00:00:00Z http://hdl.handle.net/123456789/862 Title: Structural Analysis of 4-α-Glucanotransferase from Pyrococcus Furiosus Authors: Singh, Gurkaran Mehta; Guptasarma, P.; Pandit, Shashi Bhushan Abstract: 4-α-Glucanotransferase, an enzyme from Pyrococcus furiosus, catalyzes the hydrolysis of a glucose unit from a donor molecule, and transfers it to an acceptor molecule. The sequence of this enzyme is very much similar to 4-α-Glucanotransferase from Thermococcus litoralis. The donor and acceptor molecules are carbohydrates of varying length. The donor and acceptor sites are present within the enzyme only. The donor site is present in domain-I having catalytic residues Glutamate-124 and Aspartate-215, working in a acid-base catalysis kind of mechanism, similar in case of the enzyme from Thermococcus litoralis. The acceptor site is present in domain-II having interacting residues Histidine-369 and Arginine-372. The function of domain-III is not yet known. 2017-07-18T00:00:00Z http://hdl.handle.net/123456789/861 Title: Growth of Groups Authors: Rathore, Jitendra Abstract: Let G be a finitely generated group with a finite generating set {s1, s2, ......., sn}. We define the length (l(g)) of g 2 G to be the number of generators required in the shortest decomposition of g = y1y2:::yk, where each yi is either a generator or the inverse of generator. Then we can define a metric d on G given by d(g; h) = l(gh-1). Now, if B(e; r) denotes the ball of radius r centred at identity, then define a function G(r) : N ! N given by G(r) = jB(e; r)j, which counts the size of balls. The growth rate of group is the study of the asymptotic behaviour of this function G(n). Depending on the nature of this function, we can classify the growth type into polynomial, exponential and intermediate. Here, we try to understand these growth functions and their properties. The asymptotic nature of this function provides us with a lot of information pertaining to the group. 2017-07-18T00:00:00Z